Bovine Viral Diarrhea Virus Infection

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Persistent bovine viral diarrhea virus (BVDV) infection in cattle herds

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Detection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

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ژورنال

عنوان ژورنال: Journal of the Japan Veterinary Medical Association

سال: 2012

ISSN: 0446-6454,2186-0211

DOI: 10.12935/jvma.65.111